Analytical Ultracentrifugation Laboratory
Two types of experiments are possible in the temperature range of 0 to 40 oC:
• sedimentation velocity – take about few hours (the longer the smaller object is tested),
• sedimentation equilibrium - last up to few days.
Sample volume required: 0.12 - 0.45 ml depending on the type of experiment and cuvette chosen. The required optical parameters:
• Absorption – absorbance at the selected wavelength (190 -
800 nm) in the range 0.1 – 1.5 A with optical path length 0.3 cm or 1.2 cm;
• Fluorescence - emission above 505 nm with excitation at 488 nm;
• Interference at 660 nm.
800 nm) in the range 0.1 – 1.5 A with optical path length 0.3 cm or 1.2 cm;
• Fluorescence - emission above 505 nm with excitation at 488 nm;
• Interference at 660 nm.
Experiments with interference and absorption detection may be carried out in parallel on the same sample (the same detector is used), while fluorescence detection requires an independent experiment.
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Publications based on the research performed in this NanoFun Laboratory:
- Joanna Krajewska, Anna Modrak-Wójcik, Zbigniew J. Arent, Daniel Więckowski, Michal Zolkiewski, Agnieszka Bzowska, Sabina Kędzierska-Mieszkowska, Characterization of the molecular chaperone ClpB from the pathogenic spirochaete Leptospira interrogans, PLOS ONE 12(7): e0181118. https://doi.org/10.1371/journal.pone.0181118.
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Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L. Bain, Marina M. Bakhtina, Donald F. Becker, Gregory J. Bedwell, Ahmet Bekdemir, Tabot M. D. Besong, Catherine Birck, Chad A. Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B. Chaires, Catherine T. Chaton,
Helmut Cölfen, Keith D. Connaghan, Kimberly A. Crowley, Ute Curth, Tina Daviter, William L. Dean, Ana I. Díez, Christine Ebel, Debra M. Eckert, Leslie E. Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A. Fagan, Robert Fairman, Ron M. Finn, Wolfgang Fischle, José García de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E. Harding, José G. Hernández Cifre, Andrew B. Herr, Elizabeth E. Howell, Richard S. Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A. Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A. Kusznir, Hyewon Kwon, Adam Larson, Thomas M. Laue, Aline Le Roy, Andrew P. Leech, Hauke Lilie, Karolin Luger, Juan R. Luque-Ortega, Jia Ma, Carrie A. May, Ernest L. Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J. Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K. Park, Jin-Ku Park, Peter D. Pawelek, Erby E. Perdue, Stephen J. Perkins, Matthew A. Perugini, Craig L. Peterson, Martin G. Peverelli, Grzegorz Piszczek, Gali Prag, Peter E. Prevelige, Bertrand D. E. Raynal, Lenka Rezabkova, Klaus Richter, Alison E. Ringel, Rose Rosenberg, Arthur J. Rowe, Arne C. Rufer, David J. Scott, Javier G. Seravalli, Alexandra S. Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M. Strauss, Werner W. Streicher, John P. Sumida, Sarah G. Swygert, Roman H. Szczepanowski, Ingrid Tessmer, Ronald T. Toth, IV, Ashutosh Tripathy, Susumu Uchiyama, Stephan F. W. Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H. von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E. Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M. Wubben, Peter Schuck, A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation, (2015) PloS One 10, e0126420. link -
Beata Wielgus-Kutrowska, Anna Modrak-Wójcik, Alicja Dyzma, Katarzyna Breer, Michal Zolkiewski. Agnieszka Bzowska, Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme, (2014) Archives of Biochemistry and Biophysics 549, 40-48, link
- Hou S, Trochimczyk P, Sun L, Wisniewska A, Kalwarczyk T, Zhang X, Wielgus-Kutrowska B, Bzowska A, Holyst R., How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles. Sci Rep. 2016. link
- B. Bertosa, G. Mikleusevic, B. Wielgus-Kutrowska, M. Narczyk, M. Hajnic, I. Leščić Ašler, S. Tomić, M. Luić, A. Bzowska, Homooligomerization is needed for stability: a molecular modelling and solution study of E. coli purine nucleoside phosphorylase. FEBS J, 2014. link
- Anna Modrak-Wójcik, Michał Górka, Katarzyna Niedźwiecka, Konrad Zdanowski, Joanna Żuberek, Anna Niedźwiecka and Ryszard Stolarski, Eukaryotic translation initiation is controlled by cooperativity effects within ternary complexes of 4E-BP1, eIF4E, and the mRNA 5′ cap, FEBS Letters, 2013. link
Contact:
Agnieszka Bzowska, abzowska@biogeo.uw.edu.pl, tel. 22 554 0789
Anna Modrak-Wójcik, ankam@biogeo.uw.edu.pl, tel. 22 554 0779
BeataWielgus-Kutrowska, beata@biogeo.uw.edu.pl, tel. 22 554 0789
Faculty of Physics, Division of Biophysics, University of Warsaw
Żwirki i Wigury 93
02-089 Warszawa