Cryo-SEM Laboratory


University of Warsaw, Faculty of Geology
93 Żwirki & Wigury St., 02-089 Warsaw
tel. 48 22 55 40 534, e-mail:
Head: Jerzy Trzciński


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The Laboratory is equipped with The Carl Zeiss CrossBeam Workstation AU RIGA with FIB-CryoSEM configuration and an adjunctive Sigma SEM to enter the world of 3D nanotomography. This setup combines high resolution imaging with the milling performance of the focused ion beam. We gain maximum material and topographical contrast of all kinds of samples: high resolution of up to 2.5 nm even at low acceleration voltage of 1 kV, the combined in-lens SE and BSE detector and the local charge compensation open applications in the fields of nanotomography, 3D analysis, sample preparation and nanopatterning.

• Low kV – resolution. Using low accelerating voltages you profit from less distinctive charging and shrinking effects.
• Combined in-lens SE and BSE detector. The combined in-lens SE and BSE detector delivers pure compositional and topographical contrast in addition to high resolution imaging.
• Local Charge Compensation. Eliminate charging effects of insulating materials: remove charged particles using a gas injected into the area of interest.
• EDS/EBSD analyses. Advanced analytical techniques such as high voltage material characterization by EDS and EBSD.
• Cryo preparation system. Cryogenic preparation techniques for scanning electron microscopy (SEM) are essential for the successful observation of wet or ‘beam sensitive’ specimens, such as biological tissues, cells and other environmental samples.







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Publications based on the research performed in Cryo-SEM Laboratory:

  1. Przemysław Gorzelak, Imran A. Rahman, Samuel Zamora, Arkadiusz Gąsiński, Jerzy Trzciński, Tomasz Brachaniec, Mariusz A. Salamon, Towards a Better Understanding of the Origins of Microlens Arrays in Mesozoic Ophiuroids and Asteroids, Evolutionary Biology, 2017, link
  2. Andrzej Borkowski, Łukasz Ławniczak, Tomasz Cłapa, Dorota Narożna, MarekSelwet, Daria Pęziak, Bartosz Markiewicz, Łukasz Chrzanowski, Different antibacterial activity of novel theophylline-based ionic liquids – Growth kinetic and cytotoxicity studies, Ecotoxicology and Environmental Safety 130 (2016) 54–64, link
  3. Andrzej Borkowski, Filip Owczarek, Mateusz Szala, Marek Selwet, Interaction of Gram-Positive and Gram-Negative Bacteriawith Ceramic Nanomaterials Obtained by Combustion Synthesis – Adsorption and Cytotoxicity Studies, Polish Journal of Microbiology 2016, Vol. 65, No 2, 161–170, link
  4. Andrzej Borkowski, Tomasz Cłapa, Mateusz Szala, Arkadiusz Gasinski, Marek Selwet, Synthesis of SiC/Ag/Cellulose Nanocomposite and Its Antibacterial Activity by Reactive Oxygen Species Generation, Nanomaterials 2016, 6(9), 171; doi:10.3390/nano6090171, link