Genome Analysis Laboratory

In frame of the NanoFun project, the IMB&B AMU established a Genome Analysis Laboratory, equipped with HiScanSQ and MiSeq (Illumina). This modern genomic equipment provides an integrative solution for genome-wide discovery experiments, targeted validation and screening assays on DNA and RNA level. Recently, trainings in a number of applications have been successfully completed and the following applications are available for users and collaborators:

• Small RNA sequencing;
• Whole-genome gene expression by direct hybridization;
• Custom low- to mid-plex genotyping with GoldenGate genotyping assay;
• Infinium whole-genome genotyping and copy number variation analysis;
• DNA sequencing (de novo, re-sequencing, exome sequencing, targeted re-sequencing: amplicons and TruSight Panels);
• RNA sequencing;
• CHIPseq.

We are open to collaboration and interdisciplinary projects. We offer assistance in experiment design and data analysis.  For additional information please contact Joanna Wesoły
( j.wesoly@amu.edu.pl ).

Publications:

1. E. Zodro, M. Jaroszewski, A. Ida, T. Wrzesiński, Z. Kwia, H. Bluyssen, J. Wesoly, "FUT11 as a potential biomarker of clear cell renal cell carcinoma progression based on meta-analysis of gene expression data",  Tumour Biolology, 2013. link
2. Sylwia Alaba, Pawel Piszczalka, Halina Pietrykowska, Andrzej M. Pacak, Izabela Sierocka, Przemyslaw W. Nuc, Kashmir Singh, Patrycja Plewka, Aleksandra Sulkowska, Artur Jarmolowski, Wojciech M. Karlowski and Zofia Szweykowska-Kulinska, "The liverwort Pellia endiviifolia shares microtranscriptomic traits that are common to green algae and land plants", New Phtologist, 2014. link
3. T .Wrzesiński, M. Szelag, W. A. Cieślikowski, A. Ida, R. Giles, E. Zodro, J. Szumska, J. Poźniak, Z. Kwias, H. A. Bluyssen, J. Wesoły,"Expression of pre-selected TMEMs with predicted ER localization as potential classifiers of ccRCC tumors", BMC Cancer, 2015. link