Ultrafast Microfluidic Devices Laboratory


The microscope for fast and ultrafast visualization in two and three dimensions

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  • motorized, inverted optical Eclipse Ti  microscope equipped with:
    • Prior motorized table (XY plane)
    • Epifluorescence system with laser TIRF excitation
    • External Metal-halide lamp (130 W)
  • R A1 confocal scanner
  • Set of seven lines of lasers with wavelengths:
    • 405 nm
    • 457 nm
    • 473 nm
    • 488 nm
    • 514 nm
    • 561 nm
    • 638 nm
  • FCS/FLIM System made by Picoquant containing:
    • two avalanche diode detectors
    • Pulsed laser (485 nm)

Techniques:

  • Confocal Imaging,
  • TIRF (Total Internal Reflection Fluorescence) Imaging
  • FRAP (Fluorescence Recovery after photobleaching)
  • FLIP (Fluorescence Loss in Photobleaching)
  • FRET (Förster Resonance Energy Transfer)
  • Fluorescence Correlation Spectroscopy
  • FLIM (Fluorescence Lifetime imaging microscopy)

contact: Piotr Garstecki: garst@ichf.edu.pl

 

 

 

 

 

Publications based on the research performed in this NanoFun Laboratory

  1. Hou S, Trochimczyk P, Sun L, Wisniewska A, Kalwarczyk T, Zhang X, Wielgus-Kutrowska B, Bzowska A, Holyst R., How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles. Sci Rep. 2016. link
  2. Krzysztof Sozański, Agnieszka Wiśniewska, Tomasz Kalwarczyk and Robert Hołyst. "Activation Energy for Mobility of Dyes and Proteins in Polymer Solutions: From Diffusion of Single Particles to Macroscale Flow", Physical Review Letters 111, 22, 228301. link
  3. Slawomir Jakiela, Tomasz S. Kaminski, Olgierd Cybulski, Douglas B. Weibel, and Piotr Garstecki, "Bacterial Growth and Adaptation in Microdroplet Chemostats", Angewandte Chemie, 2013. link
  4. Sen Hou, Stefan A. Wieczorek, Tomasz S. Kaminski, Natalia Ziebacz, Marcin Tabaka, Nohemy A. Sorto, Marie H. Foss, Jared T. Shaw, Martin Thanbichler, Douglas B. Weibel, Krzysztof Nieznanski, Robert Holyst and Piotr Garstecki, "Characterization of Caulobacter crescentus FtsZ Protein Using Dynamic Light Scattering", The Jouranl of Biological Chemistry, 2012. link
  5. Tomasz Kalwarczyk, Natalia Ziebacz, Anna Bielejewska, Ewa Zaboklicka, Kaloian Koynov, Jedrzej Szymanski, Agnieszka Wilk, Adam Patkowski, Jacek Gapinski, Hans-Jurgen Butt and Robert Hozyst, "Comparative Analysis of Viscosity of Complex Liquids and Cytoplasm of Mammalian Cells at the Nanoscale", Nano Letters, 2011. link
 
 
Theses based on the research performed in this NanoFun Laboratory:
1. Master thesis entitled „Zastosowanie spektroskopii korelacji fluorescencji do pomiarów energii aktywacji dyfuzji w roztworach polimerów”
 
Author: Krzysztof Sozanski
 
supervisors:  dr hab. Maciej Mazur (Faculty of Chemistry, University of Warsaw), prof. Robert Hołyst (Institute of Physical Chemistry, PAS), and dr Tomasz Kalwarczyk (Institute of Physical Chemistry, PAS)
 
2. Bachelor thesis entitled „Wykorzystanie bakteriofagów do detekcji bakterii: optymalizacja procesu przygotowania bioczujnika”
Author: Katarzyna Nasiłowska
supervisors: dr hab. Robert Hołyst (Institute of Physical Chemistry, PAS) and dr Jan Paczesny (Institute of Physical Chemistry, PAS)
3. Bachelor thesis entitled „Wyznaczanie długości micel surfaktantów przy użyciu spektroskopii korelacji fluorescencji”
Author: Monika Zapadka
supervisor: dr hab. Robert Hołyst (Institute of Physical Chemistry, PAS)
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